| Peer-Reviewed

Anti-ulcerogenic Action Mechanism of Maytenus ilicifolia Detected by mRNA RT-PCR and Free Radical Scavenging Activity Evaluated by ESR

Received: 24 February 2017     Accepted: 24 March 2017     Published: 30 March 2017
Views:       Downloads:
Abstract

Maytenus ilicifolia, known as “espinheira-santa”, is used in the popular medicine for peptic ulcer treatment. This work evaluates the preventive and curative actions of M. ilicifolia in animals, the healing activity by EGF expression detected by RT-PCR as well as the anti-oxidative activity by Electron Spin Resonance spectroscopy (ESR). Oral administration of M. ilicifolia (300 and 500 mg/kg, p.o.) one hour before the ulcerogenic agent application prevented the ulcer formation 42 ± 0.98% and 72 ± 1.2% (p <0.05 and p <0.001, respectively), in comparison with the negative control tween 80, preserving the cytoprotection characteristics of the gastric mucosa and assuring the integrity of gastric glands and gastric fossets. The animals treated with tween, had the epithelium and the mucosa layer damaged and accentuated vascularization. The healing activity of M. ilicifolia (500mg/kg, p.o.) was 71 ± 1.4% (p <0.001) in chronic ulcer experiments induced by acetic acid. EGF expression detected by RT-PCR confirmed the healing activity. Histological analysis showed the recovery of the mucosal layer and the epithelium surface harmed by the acetic acid. Studies in vitro by Electron Spin Resonance spectroscopy assessed the anti-oxidative action of M ilicifolia. Experiments with DPPH (2.2-Diphenyl -1-picrylhydrazyl) demonstrated that the polar fraction of M. ilicifolia presents an IC50 of 0.680.09 mg/ml. The value of 92 mg/ml was found for IC50 in experiments with the radical OH, produced by the Fenton Reaction and detected through spin adduct DMPO-OH. Mass spectrometry analysis of crude extracts and fractions from M. ilicifolia was carried out and compounds of pentacyclic triterpenes class were identified.

Published in American Journal of Health Research (Volume 5, Issue 3)
DOI 10.11648/j.ajhr.20170503.12
Page(s) 57-64
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Maytenus ilicifolia, Anti-oxidative Activity, Antiulcerogenic Activity, Epidermal Growth Factor, Gastric Ulcer, Electron Spin Resonance

References
[1] Rattmann YD, Cipriani TR, Sassaki GL, Iacomini M, Rieck L, Marques MCA, et al. Nitric oxide-dependent vasorelaxation induced by extractive solutions and fractions of Maytenus ilicifolia Mart ex Reissek (Celastraceae) leaves. J Ethnopharmacol. 2006; 104 (3): 328–35.
[2] Ferreira PM, de Oliveira CN, de Oliveira AB, Lopes MJ, Alzamora F, Vieira MAR. A lyophilized aqueous extract of Maytenus ilicifolia leaves inhibits histamine-mediated acid secretion in isolated frog gastric mucosa. Planta. 2004; 219 (2): 319–24.
[3] Vellosa JCR, Khalil NM, Formenton VAF, Ximenes VF, Fonseca LM, Furlan M, et al. Antioxidant activity of Maytenus ilicifolia root bark. Fitoterapia. 2006; 77 (3): 243–4.
[4] Mota da Silva L, Boeing T, Somensi LB, Cury BJ, Bispo Steimbach VM, Oliveira Silveria AC de, et al. Evidence of gastric ulcer healing activity of Maytenus robusta Reissek: In vitro and in vivo studies. J Ethnopharmacol. 2015 4; 175: 75–85.
[5] Cipriani TR, Mellinger CG, Souza LM De, Baggio CH, Freitas CS, Consuelo M, et al. Polygalacturonic acid : Another anti-ulcer polysaccharide from the medicinal plant Maytenus ilicifolia. Carbohydr Polym. 2009; 78 (2): 361–3.
[6] Jorge RM, Leite JP V, Oliveira AB, Tagliati CA. Evaluation of antinociceptive, anti-inflammatory and antiulcerogenic activities of Maytenus ilicifolia. 2004; 94: 93–100.
[7] De Paula ACB, Gracioso JS, Toma W, Hiruma-Lima CA, Carneiro EM, Brito ARMS. The antiulcer effect of Croton cajucara Benth in normoproteic and malnourished rats. Phytomedicine. 2008; 15 (10): 815–25.
[8] Wasicky A, Hernandes LS, Vetore-Neto A, Moreno PRH, Bacchi EM, Kato ETM, et al. Evaluation of gastroprotective activity of Passiflora alata. Rev Bras Farmacogn. 2015 25 (4): 407–12.
[9] Hamaishi K, Kojima R, Ito M. Anti-ulcer effect of tea catechin in rats. Biol Pharm Bull. 2006; 29 (11): 2206–13.
[10] Olfert ED, Cross BM, McWilliam A. Guide to the care and use of experimental animals. Can Counc Anim Care. 1993;
[11] Zimmermann M. Ethical guidelines for investigations of experimental pain in conscious animals. Pain. 1983; 16 (2): 109–10.
[12] Szelenyi I, Thiemer K. Distention ulcer as a model for testing of drugs for ulcerogenic side effects. Arch Toxicol. 1978; 41 (1): 99–105.
[13] Takagi K, Okabe S, Saziki R. A new method for the production of chronic gastric ulcer in rats and the effect of several drugs on its healing. Jpn J Pharmacol. 1969; 19 (3): 418–26.
[14] Konturek SJ, Brzozowski T, Majka J, Drozdowicz D, Stachura J. Adaptation of the gastric mucosa to stress. Scand J Gastroenterol. 1992; 27 (sup 193): 39–45.
[15] Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987; 162 (1): 156–9.
[16] Yokozawa T, Chen CP, Dong E, Tanaka T, Nonaka G-I, Nishioka I. Study on the inhibitory effect of tannins and flavonoids against the 1, 1-diphenyl-2-picrylhydrazyl radical. Biochem Pharmacol. 1998; 56 (2): 213–22.
[17] Pacheco A, Hespanha S, Oliveira RS. Brazilian Oral Research. 2014; 28.
[18] Miyahara MRM, Imamura PM, Freitas JC De, Leonor SJ, Baffa O, Kinoshita A, et al. Anti-oxidative and anti-ulcerogenic activity of Ipomoea imperati. Rev Bras Farmacogn. 2011; 21: 978–85.
[19] Barros Silva R, Santos NAG, Martins NM, Ferreira DAS, Barbosa Jr F, Oliveira Souza VC, et al. Caffeic acid phenethyl ester protects against the dopaminergic neuronal loss induced by 6-hydroxydopamine in rats. Neuroscience. 2013; 233 (0): 86–94.
[20] Oates PJ, Hakkinen JP. Studies on the mechanism of ethanol-induced gastric damage in rats. Gastroenterology. 1988; 94 (1): 10–21.
[21] Cutz E, Chan W, Track NS, Goth A, Said SI. Release of vasoactive intestinal polypeptide in mast cells by histamine liberators. 1978.
[22] Milani S, Calabro A. Role of growth factors and their receptors in gastric ulcer healing. Microsc Res Tech. 2001; 53 (5): 360–71.
[23] Konturek PC, Brzozowski T, Duda A, Kwiecien S, Löber S, Dembinski A, et al. Epidermal growth factor and prostaglandin E 2 accelerate mucosal recovery from stress-induced gastric lesions via inhibition of apoptosis. J Physiol. 2001; 95 (1): 361–7.
[24] Chen JH, Ho C-T. Antioxidant activities of caffeic acid and its related hydroxycinnamic acid compounds. J Agric Food Chem. 1997; 45 (7): 2374–8.
[25] Trouillas P, Calliste C-A, Allais D-P, Simon A, Marfak A, Delage C, et al. Antioxidant, anti-inflammatory and antiproliferative properties of sixteen water plant extracts used in the Limousin countryside as herbal teas. Food Chem. 2003; 80 (3): 399–407.
[26] Molyneux P. The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin J Sci Technol. 2004; 26 (2): 211–9.
[27] Ito M, Suzuki Y, Ishihara M, Suzuki Y. Anti-ulcer effects of antioxidants: effect of probucol. Eur J Pharmacol. 1998; 354 (2): 189–96.
[28] Chappman, Hall. Dictionary of Natural Products. CRC press.
[29] Queiroga CL, Silva GF, Dias PC, Possenti A, de Carvalho JE. Evaluation of the antiulcerogenic activity of friedelan-3β-ol and friedelin isolated from Maytenus ilicifolia (Celastraceae). J Ethnopharmacol. 2000; 72 (3): 465–8.
Cite This Article
  • APA Style

    Dalva Pazzini, Elder Bruno Fontes, Ana Paula D. Hory, Adevailton B. Santos, Oswaldo Baffa, et al. (2017). Anti-ulcerogenic Action Mechanism of Maytenus ilicifolia Detected by mRNA RT-PCR and Free Radical Scavenging Activity Evaluated by ESR. American Journal of Health Research, 5(3), 57-64. https://doi.org/10.11648/j.ajhr.20170503.12

    Copy | Download

    ACS Style

    Dalva Pazzini; Elder Bruno Fontes; Ana Paula D. Hory; Adevailton B. Santos; Oswaldo Baffa, et al. Anti-ulcerogenic Action Mechanism of Maytenus ilicifolia Detected by mRNA RT-PCR and Free Radical Scavenging Activity Evaluated by ESR. Am. J. Health Res. 2017, 5(3), 57-64. doi: 10.11648/j.ajhr.20170503.12

    Copy | Download

    AMA Style

    Dalva Pazzini, Elder Bruno Fontes, Ana Paula D. Hory, Adevailton B. Santos, Oswaldo Baffa, et al. Anti-ulcerogenic Action Mechanism of Maytenus ilicifolia Detected by mRNA RT-PCR and Free Radical Scavenging Activity Evaluated by ESR. Am J Health Res. 2017;5(3):57-64. doi: 10.11648/j.ajhr.20170503.12

    Copy | Download

  • @article{10.11648/j.ajhr.20170503.12,
      author = {Dalva Pazzini and Elder Bruno Fontes and Ana Paula D. Hory and Adevailton B. Santos and Oswaldo Baffa and Rosângela Bezerra and Silvana Pasetto and Eduardo José Crevelin and Luiz Alberto Beraldo Moraes and Angela Kinoshita and Ana Cláudia B. Paula-Zurron},
      title = {Anti-ulcerogenic Action Mechanism of Maytenus ilicifolia Detected by mRNA RT-PCR and Free Radical Scavenging Activity Evaluated by ESR},
      journal = {American Journal of Health Research},
      volume = {5},
      number = {3},
      pages = {57-64},
      doi = {10.11648/j.ajhr.20170503.12},
      url = {https://doi.org/10.11648/j.ajhr.20170503.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajhr.20170503.12},
      abstract = {Maytenus ilicifolia, known as “espinheira-santa”, is used in the popular medicine for peptic ulcer treatment. This work evaluates the preventive and curative actions of M. ilicifolia in animals, the healing activity by EGF expression detected by RT-PCR as well as the anti-oxidative activity by Electron Spin Resonance spectroscopy (ESR). Oral administration of M. ilicifolia (300 and 500 mg/kg, p.o.) one hour before the ulcerogenic agent application prevented the ulcer formation 42 ± 0.98% and 72 ± 1.2% (p M. ilicifolia (500mg/kg, p.o.) was 71 ± 1.4% (p in vitro by Electron Spin Resonance spectroscopy assessed the anti-oxidative action of M ilicifolia. Experiments with DPPH (2.2-Diphenyl -1-picrylhydrazyl) demonstrated that the polar fraction of M. ilicifolia presents an IC50 of 0.680.09 mg/ml. The value of 92 mg/ml was found for IC50 in experiments with the radical OH, produced by the Fenton Reaction and detected through spin adduct DMPO-OH. Mass spectrometry analysis of crude extracts and fractions from M. ilicifolia was carried out and compounds of pentacyclic triterpenes class were identified.},
     year = {2017}
    }
    

    Copy | Download

  • TY  - JOUR
    T1  - Anti-ulcerogenic Action Mechanism of Maytenus ilicifolia Detected by mRNA RT-PCR and Free Radical Scavenging Activity Evaluated by ESR
    AU  - Dalva Pazzini
    AU  - Elder Bruno Fontes
    AU  - Ana Paula D. Hory
    AU  - Adevailton B. Santos
    AU  - Oswaldo Baffa
    AU  - Rosângela Bezerra
    AU  - Silvana Pasetto
    AU  - Eduardo José Crevelin
    AU  - Luiz Alberto Beraldo Moraes
    AU  - Angela Kinoshita
    AU  - Ana Cláudia B. Paula-Zurron
    Y1  - 2017/03/30
    PY  - 2017
    N1  - https://doi.org/10.11648/j.ajhr.20170503.12
    DO  - 10.11648/j.ajhr.20170503.12
    T2  - American Journal of Health Research
    JF  - American Journal of Health Research
    JO  - American Journal of Health Research
    SP  - 57
    EP  - 64
    PB  - Science Publishing Group
    SN  - 2330-8796
    UR  - https://doi.org/10.11648/j.ajhr.20170503.12
    AB  - Maytenus ilicifolia, known as “espinheira-santa”, is used in the popular medicine for peptic ulcer treatment. This work evaluates the preventive and curative actions of M. ilicifolia in animals, the healing activity by EGF expression detected by RT-PCR as well as the anti-oxidative activity by Electron Spin Resonance spectroscopy (ESR). Oral administration of M. ilicifolia (300 and 500 mg/kg, p.o.) one hour before the ulcerogenic agent application prevented the ulcer formation 42 ± 0.98% and 72 ± 1.2% (p M. ilicifolia (500mg/kg, p.o.) was 71 ± 1.4% (p in vitro by Electron Spin Resonance spectroscopy assessed the anti-oxidative action of M ilicifolia. Experiments with DPPH (2.2-Diphenyl -1-picrylhydrazyl) demonstrated that the polar fraction of M. ilicifolia presents an IC50 of 0.680.09 mg/ml. The value of 92 mg/ml was found for IC50 in experiments with the radical OH, produced by the Fenton Reaction and detected through spin adduct DMPO-OH. Mass spectrometry analysis of crude extracts and fractions from M. ilicifolia was carried out and compounds of pentacyclic triterpenes class were identified.
    VL  - 5
    IS  - 3
    ER  - 

    Copy | Download

Author Information
  • Pró-Reitoria de Pesquisa e Pós-Gradua??o, Universidade do Sagrado Cora??o – USC, Bauru, SP, Brazil

  • Pró-Reitoria de Pesquisa e Pós-Gradua??o, Universidade do Sagrado Cora??o – USC, Bauru, SP, Brazil

  • Pró-Reitoria de Pesquisa e Pós-Gradua??o, Universidade do Sagrado Cora??o – USC, Bauru, SP, Brazil

  • Universidade Federal de Uberlandia, Uberlandia, MG, Brazil

  • Departamento de Física, FFCLRP, Universidade de S?o Paulo- USP, Ribeir?o Preto, SP, Brazil

  • Centro Universitário do Norte, UniNorte Manaus, AM, Brazil

  • Departamento de Química, FFCLRP, Universidade de S?o Paulo- USP, Ribeir?o Preto, SP, Brazil

  • Departamento de Química, FFCLRP, Universidade de S?o Paulo- USP, Ribeir?o Preto, SP, Brazil

  • Pró-Reitoria de Pesquisa e Pós-Gradua??o, Universidade do Sagrado Cora??o – USC, Bauru, SP, Brazil

  • Kroton Educacional, Valinhos, SP, Brazil

  • Sections